Generator

Part:BBa_K1060013:Design

Designed by: Ingmar Claes   Group: iGEM13_KU_Leuven   (2013-09-24)


LacI-AroG* production under regulation of TetR repressible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
    Illegal XhoI site found at 2195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1502


Design Notes

no EcoRI, XbaI, NotI, SpeI or PstI sites present


Source

Escherichia coli genome K12 (DH5alpha)

References